FR-μProbe is a miniaturized turn-key solution for optical measurements at various modes such as absorbance, transmittance, reflectance and fluorescence at the Point of Interest (PoI) through optical microscope. The tool incorporates two optical components: a spectrometer operating in the spectral range supported by the microscope and an adaptor for the eye lens of the optical microscope. The light source used for the measurements is the one available in the optical microscope or an external one. The adaptor for the eye lens is tailored to the dimensions of the microscope to be mounted on, and include a lens for increased light collection. The size area from which the signal is collected is defined by the magnification of the objective lens used. Typically for a 40X objective lens, the monitored area is a circle with ~ 200μm diameter. The collection area could be further decreased by using objective lenses of higher magnification.

Furthermore through processing of reflectance measurements the film thickness and optical constants (n & k) of the areas of thin and thick film stacks under investigation are calculated
FR-μProbe case is designed and fabricated with Anodized Aluminum profile and offers an elegant and robust construction.

Fig. 1: FR-μProbe installed on a optical microscope using the internal light source. Fig. 2: Typical measurement of a thick SiO2 film through an X20 objective of a microscope, using the FR-Monitor software.

The system can be easily used by anyone with basic computer skills without any deep knowledge of optics and requires a PC with a USB port available.


FR-Monitor® is used for the tool control and processing of the spectral measurements and offers unique capabilities for a wide range of applications and versatility. It acquires in real time absorbance, transmittance, reflectance, or fluorescence spectra, and performs very fast computations thanks to the state of the art algorithms implemented in Visual C++.

In absorbance / transmittance configuration, all typical parameters such as A, T are calculated. Furthermore, other non-conventional parameters such as signal integration that are very useful in other measurements are calculated.

In the case of reflectance measurements, FR-Monitor the software includes the White Light Reflectance Spectroscopy (WLRS) algorithm (ThetaMetrisisTM) for accurate calculation of film thickness (<10nm up to 100μm) and optical constants (n & k) of free-standing and supported (over transparent or partially/fully reflective substrates) stack of (<10 layers) films.



10nm - 150μm*
Wavelength range
Chemo-Bio Sensing
Chemical measurements
better than 0.5 %
Materials characterization
Spot size
depends on objective's magnification
Light Source
Microscope's source / External Tungsten - Halogen (>360nm)
Polymer Characterization
Sample size
10 - 150mm, irregular shape
Optical Coating
Computer requirements
PC with Windows XP/Vista/7 32/64bit and a USB port available
In-Situ Measurements
Power requirements
110V/230V AC (if external light source is used)
Dimensions (WxLxH)
12cm x 12cm x 10cm*
* Depends on the configuration, please refer to FR-Basic page.
**Specifications are subject to change without any notice
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